精製ニワトリ・オボムコイドの焦点電気泳動

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  • 精製ニワトリ・オボムコイドの焦点電気泳動(農芸化学部門)
  • セイセイ ニワトリ オボムコイド ノ ショウテン デンキ エイドウ
  • Isoelectrofocusing of purified chicken ovomucoid (Agricultural Chemistry)

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Abstract

The purified chicken ovomucoid was further fractionated with the method of isoelectrofocusing. The ovomucoid was fractionated into six fractions having the pI value of 3.52,3.82,4.00,4.15,4.35 and 4.50. The peak of protein with the pI value of 3.52 was not detected in the deflavo-ovomucoid. The trypsin inhibitory activity which was considered to be one of the biological activity of ovomucoid was detected in the fractions of pI value of 3.52,3.85,4.00 and 4.15,and the proteins of pI value of 4.35 and 4.50 did not show the trypsin inhibitory activity. Contrary to the results above mentioned, the flavin binding activity occurred in the peaks of pI value of 4.15,4.35 and 4.50,and it was not observed in the other peaks. The apparatus for the isoelectrofocusing used in these experiments was designed and prepared in our laboratory. The satisfactory and reproducible results were obtained with these equipments.

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