クロレラからの血圧降下作用糖蛋白質の検索

IR JASI Web Site

Bibliographic Information

Other Title
  • クロレラからの血圧降下作用糖蛋白質の検索(農芸化学部門)
  • クロレラ カラ ノ ケツアツ コウカ サヨウ トウ タンパクシツ ノ ケンサク
  • Detection of antihypertensive glycoprotein from chlorella (Agricultural Chemistry)

Search this article

Abstract

The antihypertensive material was partially purified from chlorella. Cell wall of chlorella was destroyed with Dyno-mill. After defatted and decolored with cold aceton, water extract was obtained from chlorella by the extraction at 4℃ for 24 hours. 80% ethanol precipitate from water extract was fractionated by Bio-Gel P-60 gel filtration followed by DEAE-cellulose chromatatography. About 50mg of antihypertensive fraction D-II were obtained from 10g of chlorella. The molecular weight of fraction D-II was about 80,000 dalton. The blood pressure of spontaneously hypertensive rat (13 weeks of age) was decreased from 185 to 150mmHg, when fraction D-II was injected intraperitoneally at the concentration of 0.5mg/100g of body weight. Fraction D-II contained galactose, mannose and glucosamine. The contents of aspartic acid, glutamic acid, alanine and proline in fraction D-II were large.

Journal

Details 詳細情報について

Report a problem

Back to top