Studies on Tissue Culture of Vigna sinensis Endl. with Reference to Callus Formation, Subculture and Habituation (A. NATURAL SCIENCE)

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  • ササゲ(Vigna sinensis)の組織培養におけるカルス形成,継代培養および馴化について〔英文〕
  • ササゲ Vigna sinensis ノ ソシキ バイヨウ ニ オケル カルス
  • ササゲ (Vigna sinensis) の組織培養におけるカルス形成, 継代培養および馴化について(A. 理学)

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Abstract

Tissue culture of Vigna sinensis Endl. was studied with reference to callus formation, subculture and habituation. Callus formation was observed on cotyledon, hypocotyl and root sections, when cultured on Linsmaier and Skoog basal medium supplemented with IAA or 2,4-D. 2,4-D showed higher stimulative activity than IAA. Cotyledon sections required higher auxin level for callus formation than hypocotyl and root ones. Kinetin and nicotinic acid were not so effective to the callus formation. Yeast extract caused vigorous callus formation, when used with auxin and kinetin. No bud formation was observed in any section on any medium. In the subculture of the callus, IAA (10^<-7>∿4×10^<-5>M) gave brown callus with roots, possibly containing differentiated tracheal elements, while 2,4-D (except 10^<-8> and 10^<-9>M) did yellow friable and homogenous callus without root. Kinetin somewhat promoted the callus growth, and significantly affected the tissue texture. High level of kinetin gave compact callus, while high level of auxin did friable callus. Nicotinic acid promoted much the callus growth, but had no effect on the tissue texture. Yeast extract strongly stimulated the callus growth. After more than 15 transfers of subculture in the media added with 2,4-D, kinetin, nicotinic acid and yeast extract, alone or in combination, at least three distinguishable stock calli with different requirement for growth factors were obtained.

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