書誌事項
- タイトル別名
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- キーウィフルーツ・プロテアーゼの分離・精製--新しいイオン交換充填剤Bakerbond WP-PEIによる分離
- キーウィ フルーツ プロテアーゼ ノ ブンリ セイセイ アタラシイ イオン コ
- Separation and Purification of Kiwifruit Protease : Purification with Bakerbond WP-PEI (B. LIVING SCIENCE)
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説明
Kiwifruit protease (EC 3. 4. 22. 14) was purified with conventional DEAE-cellulose and Octyl-Sepharose, a packing material of hydrophobic chromatography. With these methods, a rapid purification or concentration of the enzyme was obtained. The partially purified protease was further purified and successfully separated on the column of Bakerbond WP-PEI with a lineargradient elution of 0-0.75M NaCl into two enzymes, A_1 and A_2,which were named by Mcdowall (Biochim. Biophys. Acta, 293,226 (1973)). Kiwifruit proteases A_1 and A_2 were purified to 90% and 93% homogeneity of mol. wt. as checked by TSK G2000SW XL gel-chromatography.
収録刊行物
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- 京都府立大学学術報告. 理学・生活科学
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京都府立大学学術報告. 理学・生活科学 44 37-41, 1993-11-30
京都 : 京都府立大学学術報告委員会
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詳細情報 詳細情報について
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- CRID
- 1050001337626990336
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- NII論文ID
- 110000058177
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- NII書誌ID
- AN00062300
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- NDL書誌ID
- 3601356
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- ISSN
- 0075739X
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- 本文言語コード
- ja
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- 資料種別
- departmental bulletin paper
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- データソース種別
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- IRDB
- NDL
- CiNii Articles