Morphological and Cytochemical Characteristics of Neutrophil from Polypterus endlicheri

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  • ポリプテルス好中球の形態学的および細胞化学的特徴
  • ポリプテルスコウチュウキュウ　ノ　カイタイガクテキ　オヨビ　サイボウカガクテキトクチョウ

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Abstract

Morphological and cytochemical characteristics of neutrophil in Polypterus endlicheri were examined by light microscopy and the composition of neutrophil granule were inferred in this study. The neutrophils were round or oval(14 .0 ～ 23.0 μm in diameter) and the nucleus round to two lobule-shaped. Three types of granules； two types of acidophilic granules (α-1G and α-2G), and basophilic granule (γG) were observed in neutrophil. Chromophobic granule (βG) was not observed. The α-1G was round (0.3μm in diameter) or rod (0.8μm in length) in shape and observed in May-Grünwald (MG), Giemsa and MG-Giemsan (MGG) preparations. The α-1G was alkaline phosphatase, acid phosphatase, β-glucronidase, α-naphtyl acetate esterase, α-naphtyl butyrate esterase, naphthol AS-D chloroacetate esterase and sudan black B positive. On the preparation fixed with formalin vapor, the α-1G was stained with eosin. The α-2G was round (0.3μm in diameter) and observed in MG and MGG preparations using 1/15M phosphate buffer (pH7.0 and pH8.0). The α-2G was stained with eosin on the preparation fixed with formalin-methanol. The γG was round to oval (<0.5μm in diameter) and stained with Giemsa and MGG using 1/15M phosphate buffer (pH7.0 and pH8.0). Yasumoto-body (Y-body) was also observed in neutrophil and stained with toluidine blue, methylene blue, azure A and safranine O.

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