悪臭成分の分析とﾒﾀﾝﾁｵｰﾙ分解菌の特徴

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  • Analyzing Malodorous Compounds and Characteristics of Methanethiol Decomposing Bacteria

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Methanethiol is a corrosive gas with a strong odor, and has the poisoning action resembled to hydrogen sulfide. It has the environmental toxicity and is soluble in water; therefore, it is very important to develop an easily measurable method for methanethiol in aqueous solutions. In this series of research, a fast and convenient analytical method was developed and applied to determine the concentration of methanethiol in aqueous solutions. It is based on the Ellman reaction, a reaction of methanethiol with 5,5'-dithiobis(2-nitrobenzoic acid)(DTNB), and the measurement of the absorbance of the product, namely quinoid anion, at 412 nm. The concentration of methanethiol was directly proportional to the absorbance at 412 nm, and a linear standard calibration curve was obtained in a certain range of concentration. In this research, an applicability of the new measurement method for the screening of methanethiol degrading bacteria was evaluated and it was found to be effective for measuring methanethiol in aqueous solution. As a result of the screening, TOS-35(Alcaligenes faecalis)demonstrated the highest ability of decomposing methanethiol and two feasibility studies were done in order to test the effectiveness of this bacterium.

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