食用キノコのスフィンゴ脂質 : Ⅰ. エリンギ, Pleurotus eryngii の遊離セラミドおよびセレブロシド

書誌事項

タイトル別名
  • ショクヨウ キノコ ノ スフィンゴ シシツ 1 エリンギ Pleurotus eryngii ノ ユウリ セラミド オヨビ セレブロシド
  • Studies on Sphingolipids of Edible Fungi : Ⅰ. Characterizaion of Free Ceramides and Cerebrosides in Pleurotus eryngii
公開日
2003
資源種別
departmental bulletin paper
公開者
滋賀大学教育学部

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説明

Free ceramides(Cer-1 and Cer-2) and cerebrosides(glucocerebrosides,CMH-1 and CMH-2) were isolatated from the Eringi mushroom, Pleurotus eryngii by successive column chromatography on ion exchange Sephadex(QAE-Sephadex), magnesium silicate(Florisil) and silicic acid(Iatrobeads). By gas-liquid chromatography(GC), gas chromatograph-mass spectrometry(GC-MS) and matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF MS), their structures were characterized as ;Cer-1 : N-palmitoyl-(16.7%), N-stearoyl-(14.0%), N-behenoyl-(19.2%) and N-lignoceroyl-phytosphingosines(27.7%); Cer-2 : N-2'-hydroxybehenoyl-(22.6%), and N-2'-hydroxylignoceroyl-phytosphingosines(60.5%); CMH-1 : 1-O-glucosyl-N-2'-hydroxypentadecanoyl-(13.8%) and 1-O-glucosyl-N-2'-hydroxypentadecanoyl-nonadecasphingadienines(71.2%); and CMH-2 : 1-O-glucosyl-N-2'-hydroxypentadecanoyl-(15.3%) and 1-O-glucosyl-N-2'-hydroxypalmitoyl-phytosphingosines(84.7%)

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