Chemical hijacking of auxin signaling with an engineered auxin–TIR1 pair

Uchida, Naoyuki, Takahashi, Koji, Iwasaki, Rie, Yamada, Ryotaro, Yoshimura, Masahiko, Endo, Takaho A, Kimura, Seisuke, Zhang, Hua, Nomoto, Mika, Tada, Yasuomi, Kinoshita, Toshinori, Itami, Kenichiro, Hagihara, Shinya, Torii, Keiko U

doi:10.1038/nchembio.2555

The phytohormone auxin indole-3-acetic acid (IAA) regulates nearly all aspects of plant growth and development. Despite substantial progress in our understanding of auxin biology, delineating specific auxin response remains a major challenge. Auxin regulates transcriptional response via its receptors, TIR1 and AFB F-box proteins. Here we report an engineered, orthogonal auxin–TIR1 receptor pair, developed through a bump-and-hole strategy, that triggers auxin signaling without interfering with endogenous auxin or TIR1/AFBs. A synthetic, convex IAA (cvxIAA) hijacked the downstream auxin signaling in vivo both at the transcriptomic level and in specific developmental contexts, only in the presence of a complementary, concave TIR1 (ccvTIR1) receptor. Harnessing the cvxIAA–ccvTIR1 system, we provide conclusive evidence for the role of the TIR1-mediated pathway in auxin-induced seedling acid growth. The cvxIAA–ccvTIR1 system serves as a powerful tool for solving outstanding questions in auxin biology and for precise manipulation of auxin-mediated processes as a controllable switch.

Chemical hijacking of auxin signaling with an engineered auxin–TIR1 pair

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Chemical hijacking of auxin signaling with an engineered auxin–TIR1 pair

この論文をさがす

説明

収録刊行物

被引用文献 (28)*注記

参考文献 (42)*注記

関連プロジェクト

キーワード

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書き出し

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