海水より分離した寒天分解菌について

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  • カイスイ ヨリ ブンリ シタ カンテン ブンカイキン ニ ツイテ
  • Studies on the Agar-decomposing Bacteria Isolated from Sea-water

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Abstract

The authors isolated two strains of agar-decomposing bacteria from the surface of Porphyra tenera, and studied on the optimum conditions to the growth and agar-liquefying capacity of these bacteria, and the general properties of crude agar-decomposing enzyme prepared from the bacterial culture, was also examined. And the following results were obtained. 1) The isolated bacteria Bact-5 and Bact-7 seemed to be a sort of Flavobac-terium and Pseudomonas, respectively. 2) Agar-liquefying by these bacteria was accomplished utmostly under follwing conditions,—at 25°-30°C; in the medium containing 3 of NaC1 and NaNO3 as nitrogen source; pH 5.8—'7.0. And the activity reached to the maximum state at about 113 hours. 3) The effects of pH value and temperature on the activity of the crude agar-decomposing enzyme were studied by measuring the decreasing rate of relative viscosity of agar solution, and the most powerful activity was shown at pH 5.6 and 40°C. 4) Liquefaction of agar-agar by the crude enzyme reached to the state of equilibrium between 3-.-5 hours from beginning of the action, but production of reducing sugar was late for a long hours.

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