Effects of non-B DNA sequences on transgene expression
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説明
DNA conformation may be an important factor affecting gene transcription. In this study, we examined how DNA sequences with unusual conformations affect transgene expression. A30 and (CG)15 sequences that can adopt the B' and Z conformations, respectively, were introduced into a β-actin promoter. Luciferase plasmids containing the manipulated promoter were transfected into NIH3T3 cells by electroporation and were delivered into mouse livers with a hydrodynamics-based injection. Expression from plasmid with the (CG)15 sequence was multiple times higher than expression from control plasmid DNA. The A30 sequence also tended to enhance expression. These results suggest that non-B DNA sequences could improve transgene expression in cells.
収録刊行物
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- Journal of Bioscience and Bioengineering
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Journal of Bioscience and Bioengineering 108 (1), 20-23, 2009-07
The Society for Biotechnology, Japan
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詳細情報 詳細情報について
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- CRID
- 1050001339001018240
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- NII論文ID
- 110007359709
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- NII書誌ID
- AA11307678
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- ISSN
- 13474421
- 13891723
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- HANDLE
- 2115/38996
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- NDL書誌ID
- 10299659
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- IRDB
- NDL
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- CiNii Articles
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