Effects of Chronic Alcohol Use Mouse Myocardial Tissue

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  • Effects of Chronic Alcohol Use on Mouse Myocardial Tissue

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Alcohol intake induces cardiovascular effects. We performed a comprehensive analysis of the gene expression profiles of myocardial tissues from a mouse model of alcohol feeding by microarray to clarify the effect of alcohol on myocardium from the perspective that the acute phase of alcohol use causes changes to myocardial injury related gene expression profiles, while the withdrawal phase is associated with changes in gene expression profiles related to myocardial protection. A model of single alcohol feeding was generated using pathogen-free, 7-weekold, male C57BL/6N mice administered a peroral injection of 7.0 g/kg ethanol (single alcohol-fed group group) or saline (control group). A repeated alcohol feeding group was generated following Lieber's method. To investigate the effects of alcohol in the alcohol-fed group, the left ventricles of hearts were extirpated 1h(alcohol group) or 24h(withdrawal group) after the last alcohol feeding. Myocardial tissues were then subjected to RNA extraction and gene expression analysis by real-time reverse transcription polymerase chain reaction. Sera were also analyzed for various signal transducers, including 22 cytokines. Using RNA extracted from extirpated myocardia, mRNA expressions were comprehensively analyzed by one-color microarray (n=4) using SurePrint G3 Mouse Gene Expression 8x60K (Agilent) containing 38,64O gene probes as the microchip, and gene ontology and gene cascade analyses were performed. Differing gene profiles were seen in the repeated alcohol intake group. In the acute phase of alcohol intake, there were changes in gene profiles related to activation of the STAT pathway and blood cytokine-mediated inflammatory responses to hypercardia. In the withdrawal from alcohol phase, changes in gene profiles related to STAT activity did not persist, although gene profile changes related to apoptosis and the development of sudden death were observed. Further detailed investigations of the effects of alcohol on cardiomyocytes are planned.

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