Development of a High‐Pressure Reactor Based on Liquid‐Flow Pressurisation to Facilitate Enzymatic Hydroxylation of Gaseous Alkanes

DOI DOI IR IR (HANDLE) Web Site View 3 Remaining Hide 15 Citations 18 References Open Access

Bibliographic Information

Other Title
  • Development of a high-pressure reactor based on liquid-flow pressurisation to facilitate enzymatic hydroxylation of gaseous alkanes

Search this article

Description

A new type of high‐pressure reactor based on liquid‐flow pressurisation using a HPLC pump has been developed. This high‐pressure reactor allows the easy and safe performance of reactions with gaseous alkanes under high‐pressures up to 10 MPa (100 atm), without the need for high‐pressure gas cylinders. The amount of substrate gas required for a single reaction is very small compared with reactions using a conventional autoclave, which, when using expensive substrate gasses, such as 13C‐labelled ethane, becomes critical. Employing this high‐pressure reactor in conjunction with cytochrome P450BM3 and the assistance of decoy molecules, the direct hydroxylation of gaseous alkanes was drastically improved. At 5 MPa the TOF of propane hydroxylation increased 10‐fold, reaching 2200 min^−1. Hydroxylation of ethane was also substantially accelerated at 5 MPa, reaching a TOF of 28 min^−1.

Journal

  • ChemCatChem

    ChemCatChem 11 (19), 4709-4714, 2019-10-07

    Wiley

Citations (15)*help

See more

References(18)*help

See more

Related Projects

See more

Keywords

Report a problem

Back to top