RNA-Seq reveals virus-virus and virus-pant interactions in nature
-
- Rolf Kümmerli
- editor
書誌事項
- タイトル別名
-
- RNA-Seq reveals virus–virus and virus–plant interactions in nature
この論文をさがす
説明
As research on plant viruses has focused mainly on crop diseases, little is known about these viruses in natural environments. To understand the ecology of viruses in natural systems, comprehensive information on virus–virus and virus–host interactions is required. We applied RNA-Seq to plants from a natural population of Arabidopsis halleri subsp. gemmifera to simultaneously determine the presence/absence of all sequence-reported viruses, identify novel viruses and quantify the host transcriptome. By introducing the criteria of read number and genome coverage, we detected infections by Turnip mosaic virus (TuMV), Cucumber mosaic virus and Brassica yellows virus. Active TuMV replication was observed by ultramicroscopy. De novo assembly further identified a novel partitivirus, Arabidopsis halleri partitivirus 1. Interestingly, virus reads reached a maximum level that was equivalent to that of the host's total mRNA, although asymptomatic infection was common. AhgAGO2, a key gene in host defence systems, was upregulated in TuMV-infected plants. Multiple infection was frequent in TuMV-infected leaves, suggesting that TuMV facilitates multiple infection, probably by suppressing host RNA silencing. Revealing hidden plant–virus interactions in nature can enhance our understanding of biological interactions and may have agricultural applications.
収録刊行物
-
- FEMS Microbiology Ecology
-
FEMS Microbiology Ecology 92 (11), fiw176-, 2016-11
Oxford University Press (OUP)
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1050003824756420864
-
- NII論文ID
- 120006840167
-
- ISSN
- 15746941
- 01686496
-
- HANDLE
- 2433/250508
-
- PubMed
- 27549115
-
- 本文言語コード
- en
-
- 資料種別
- journal article
-
- データソース種別
-
- IRDB
- Crossref
- CiNii Articles
- KAKEN
- OpenAIRE