Production of monoclonal antibodies against citrinin

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  • シトリニンに対するモノクローナル抗体の作製
  • シトリニン ニ タイスル モノクローナル コウタイ ノ サクセイ

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Citrinin (CIT) is a nephrotoxic mycotoxin that contaminates barley, cheese, and Monascus color. In Japan, there is the regulation that the CIT concentration in Monascus color is less than 200 µg/kg. Therefore, we prepared anti-CIT monoclonal antibody for the purpose of developing a highly sensitive and simple analyzed method for CIT in these. Twelve BALB/c mice and 12 CB6F1 mice were immunized with CIT-KLH three times each, and as a result, an increase in the antibody titer against CIT was confirmed in each of the one mouse. The mouse spleen cells were fused by standard methods. After HAT selection and cloning, we obtained stable anti-CIT monoclonal antibody-producing hybridomas CIT.6 and CIT.7. Optimized competitive indirect ELISA using CIT.6 antibody was able to detect CIT up to 0.3 µg/mL. It is expected that a highly sensitive and simple analyzed method for CIT in foods will be established.

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