Mannan endo-1,4-β-mannosidase from Kitasatospora sp isolated in Indonesia and its potential for production of mannooligosaccharides from mannan polymers

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抄録

Mannan endo-1,4-β-mannosidase(commonly known as β-mannanase) catalyzes a random cleavage of the β-D-1,4-mannopyranosyl linkage in mannan polymers. The enzyme has been utilized in biofuel production from lignocellulose biomass, as well as in production of mannooligosaccharides (MOS) for applications in feed and food industries. We aimed to obtain a β-mannanase, for such mannan polymer utilization, from actinomycetes strains isolated in Indonesia. Strains exhibiting high mannanase activity were screened, and one strain belonging to the genus Kitasatospora was selected. We obtained a β-mannanase from this strain, and an amino acid sequence of this Kitasatospora β-mannanase showed a 58-71% similarity with the amino acid sequences of Streptomyces β-mannanases. The Kitasatospora β-mannanase showed a significant level of activity (944 U/mg) against locust bean gum (0.5% w/v) and a potential for oligosaccharide production from various mannan polymers. The β-mannanase might be beneficial particularly in the enzymatic production of MOS for applications of mannan utilization.

収録刊行物

  • AMB Express

    AMB Express 7 100-100, 2017-05-19

    SpringerOpen

詳細情報 詳細情報について

  • CRID
    1050012570393965312
  • NII論文ID
    120006318802
  • ISSN
    21910855
  • HANDLE
    20.500.14094/90004050
  • 本文言語コード
    en
  • 資料種別
    journal article
  • データソース種別
    • IRDB
    • CiNii Articles

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