Cyclic Tau-derived peptides for stabilization of microtubules
この論文をさがす
抄録
The cyclization of peptides is a valuable strategy for the development of binding motifs to target proteins with improved affinity. Microtubules (MTs) are important targets for therapeutics, and a variety of MT-targeted drugs and peptides have recently been developed. We have previously designed a Tau-derived peptide (TP) that binds to the interior of MTs. In the present study, the development of a cyclic TP (TCP) for enhanced binding to tubulin and the stabilization of MTs is described. The fluorescently labeled cyclic peptide containing three glycine linkers (TCP3-TMR) exhibited a remarkably enhanced binding affinity to tubulin. The cyclic peptide was also demonstrated to stabilize MTs by enhancing polymerization and reducing depolymerization. Moreover, MTs were effectively formed by the treatment of cyclic peptides in the presence of guanosine triphosphate (GTP), while the linear peptide showed no such effect. These findings indicate that TCP is a useful binding motif that can stabilize MTs and is valuable for various therapeutic and material applications.
収録刊行物
-
- POLYMER JOURNAL
-
POLYMER JOURNAL 52 (9), 1143-1151, 2020-09
Springer Nature
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1050015354550285312
-
- NII論文ID
- 40022335739
-
- NII書誌ID
- AA00777013
-
- ISSN
- 00323896
- 13490540
-
- NDL書誌ID
- 030613945
-
- 本文言語コード
- en
-
- 資料種別
- journal article
-
- データソース種別
-
- IRDB
- NDL
- Crossref
- CiNii Articles
- KAKEN