Transcriptionally linked simultaneous overexpression of P450 genes for broad-spectrum herbicide resistance
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- Suda, Hiroe
- Graduate School of Agriculture, Kyoto University
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- Kubo, Tomomi
- Graduate School of Agriculture, Kyoto University
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- Yoshimoto, Yusuke
- Graduate School of Agriculture, Kyoto University
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- Tanaka, Keisuke
- NODAI Genome Research Center, Tokyo University of Agriculture
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- Tanaka, Satoru
- Faculty of Regional Environment Science, Tokyo University of Agriculture
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- Uchino, Akira
- Central Region Agricultural Research Center, National Agriculture and Food Research Organization
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- Azuma, Satoshi
- Crop Research Center, Niigata Agricultural Research Institute
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- Hattori, Makoto
- Crop Research Center, Niigata Agricultural Research Institute
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- Yamaguchi, Takuya
- Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University
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- Miyashita, Masahiro
- Graduate School of Agriculture, Kyoto University
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- Tominaga, Tohru
- Graduate School of Agriculture, Kyoto University
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- Iwakami, Satoshi
- Graduate School of Agriculture, Kyoto University
Abstract
Broad-spectrum herbicide resistance (BSHR), often linked to weeds with metabolism-based herbicide resistance, poses a threat to food production. Past studies have revealed that overexpression of catalytically promiscuous enzymes explains BSHR in some weeds; however, the mechanism of BSHR expression remains poorly understood. Here, we investigated the molecular basis of high-level resistance to diclofop-methyl in BSHR late watergrass (Echinochloa phyllopogon) found in the United States, which cannot be solely explained by the overexpression of promiscuous cytochrome P450 monooxygenases CYP81A12/21. The BSHR late watergrass line rapidly produced 2 distinct hydroxylated diclofop acids, only 1 of which was the major metabolite produced by CYP81A12/21. RNA-seq and subsequent reverse transcription quantitative PCR (RT-qPCR)-based segregation screening identified the transcriptionally linked overexpression of a gene, CYP709C69, with CYP81A12/21 in the BSHR line. The gene conferred diclofop-methyl resistance in plants and produced another hydroxylated diclofop acid in yeast (Saccharomyces cerevisiae). Unlike CYP81A12/21, CYP709C69 showed no other herbicide-metabolizing function except for a presumed clomazone-activating function. The overexpression of the 3 herbicide-metabolizing genes was also identified in another BSHR late watergrass in Japan, suggesting a convergence of BSHR evolution at the molecular level. Synteny analysis of the P450 genes implied that they are located at mutually independent loci, which supports the idea that a single trans-element regulates the 3 genes. We propose that transcriptionally linked simultaneous overexpression of herbicide-metabolizing genes enhances and broadens the metabolic resistance in weeds. The convergence of the complex mechanism in BSHR late watergrass from 2 countries suggests that BSHR evolved through co-opting a conserved gene regulatory system in late watergrass.
Journal
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- Plant Physiology
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Plant Physiology 192 (4), 3017-3029, 2023-08
Oxford University Press (OUP)
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Details 詳細情報について
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- CRID
- 1050015564874782848
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- ISSN
- 15322548
- 00320889
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- HANDLE
- 2433/284587
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- Text Lang
- en
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- Article Type
- journal article
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- Data Source
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- IRDB