A Proximity‐Induced Fluorogenic Reaction Triggered by Antibody–Antigen Interactions with Adjacent Epitopes

HANDLE
  • Nishiyama, Kentaro
    Graduate School of Pharmaceutical Sciences, Kyoto University
  • Akiba, Hiroki
    Graduate School of Pharmaceutical Sciences, Kyoto University; National Institutes of Biomedical Innovation, Health and Nutrition
  • Nagata, Satoshi
    National Institutes of Biomedical Innovation, Health and Nutrition
  • Tsumoto, Kouhei
    National Institutes of Biomedical Innovation, Health and Nutrition; School of Engineering, The University of Tokyo
  • Kamada, Haruhiko
    Graduate School of Pharmaceutical Sciences, Kyoto University; National Institutes of Biomedical Innovation, Health and Nutrition
  • Ohno, Hiroaki
    Graduate School of Pharmaceutical Sciences, Kyoto University; National Institutes of Biomedical Innovation, Health and Nutrition

Abstract

Proximity-induced chemical reactions are site-specific and rapid by taking advantage of their high affinity and highly selective interactions with the template. However, reactions induced solely by antibody–antigen interactions have not been developed. Herein, we propose a biepitopic antigen-templated chemical reaction (BATER) as a novel template reaction. In BATER, reactive functional groups are conjugated to two antibodies that interact with two epitopes of the same antigen to accelerate the reaction. We developed a method for visualizing the progress of BATER using fluorogenic click chemistry for optimal antibody selection and linker design. The reaction is accelerated in the presence of a specific antigen in a link-er length-dependent manner. The choice of the antibody epitope is important for a rapid reaction. This design will lead to various applications of BATER in living systems.

Journal

Related Projects

See more

Keywords

Details 詳細情報について

  • CRID
    1050015952261745280
  • ISSN
    15213773
    14337851
  • HANDLE
    2433/284944
  • Text Lang
    en
  • Article Type
    journal article
  • Data Source
    • IRDB

Report a problem

Back to top