The phosphorylated pathway of serine biosynthesis affects sperm, embryo, and sporophyte development, and metabolism in Marchantia polymorpha
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- Wang, Mengyao
- RIKEN Center for Sustainable Resource Science; Graduate School of Bioagricultural Sciences, Nagoya University
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- Tabeta, Hiromitsu
- RIKEN Center for Sustainable Resource Science; Graduate School of Arts and Sciences, The University of Tokyo; Department of Biology, Tokyo Gakugei University
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- Ohtaka, Kinuka
- RIKEN Center for Sustainable Resource Science; Graduate School of Bioagricultural Sciences, Nagoya University; Present address: Department of Chemical and Biological Sciences, Japan Women’s University
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- Kuwahara, Ayuko
- RIKEN Center for Sustainable Resource Science
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- Nishihama, Ryuichi
- Graduate School of Biostudies, Kyoto University; Present address: Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science
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- Ishikawa, Toshiki
- Graduate School of Science and Engineering, Saitama University
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- Toyooka, Kiminori
- RIKEN Center for Sustainable Resource Science
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- Sato, Mayuko
- RIKEN Center for Sustainable Resource Science
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- Wakazaki, Mayumi
- RIKEN Center for Sustainable Resource Science
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- Akashi, Hiromichi
- RIKEN Center for Sustainable Resource Science
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- Tsugawa, Hiroshi
- RIKEN Center for Sustainable Resource Science; Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
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- Shoji, Tsubasa
- RIKEN Center for Sustainable Resource Science
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- Okazaki, Yozo
- RIKEN Center for Sustainable Resource Science; Graduate School of Bioresource, Mie University
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- Yoshida, Keisuke
- Institute of Innovative Research, Tokyo Institute of Technology
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- Sato, Ryoichi
- RIKEN Center for Sustainable Resource Science
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- Ferjani, Ali
- Department of Biology, Tokyo Gakugei University
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- Kohchi, Takayuki
- Graduate School of Biostudies, Kyoto University
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- Hirai, Masami Yokota
- RIKEN Center for Sustainable Resource Science; Graduate School of Bioagricultural Sciences, Nagoya University
Abstract
Serine metabolism is involved in various biological processes. Here we investigate primary functions of the phosphorylated pathway of serine biosynthesis in a non-vascular plant Marchantia polymorpha by analyzing knockout mutants of MpPGDH encoding 3-phosphoglycerate dehydrogenase in this pathway. Growth phenotypes indicate that serine from the phosphorylated pathway in the dark is crucial for thallus growth. Sperm development requires serine from the phosphorylated pathway, while egg formation does not. Functional MpPGDH in the maternal genome is necessary for embryo and sporophyte development. Under high CO2 where the glycolate pathway of serine biosynthesis is inhibited, suppressed thallus growth of the mutants is not fully recovered by exogenously-supplemented serine, suggesting the importance of serine homeostasis involving the phosphorylated and glycolate pathways. Metabolomic phenotypes indicate that the phosphorylated pathway mainly influences the tricarboxylic acid cycle, the amino acid and nucleotide metabolism, and lipid metabolism. These results indicate the importance of the phosphorylated pathway of serine biosynthesis in the dark, in the development of sperm, embryo, and sporophyte, and metabolism in M. polymorpha.
Journal
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- Communications Biology
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Communications Biology 7 2024-01-24
Springer Nature
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Keywords
Details 詳細情報について
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- CRID
- 1050017500391720576
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- ISSN
- 23993642
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- HANDLE
- 2433/286779
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- Text Lang
- en
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- Article Type
- journal article
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- Data Source
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- IRDB