Estimation of CO2 partial pressure and bicarbonate concentration in the hemolymph of the noble scallop Mimachlamys nobilis

半田, 岳志, 山元, 憲一

Estimation of CO2 partial pressure and bicarbonate concentration in the hemolymph of the noble scallop Mimachlamys nobilis

半田, 岳志

水産研究・教育機構
山元, 憲一

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説明

We examined hemolymph pH, total CO2 content (Tco2, mM/l), CO2 partial pressure (Pco2, mmHg) and bicarbonate concentration ([HCO3- ], mM/l) in order to evaluate the acid-base balance of the noble scallop Mimachlamys nobilis in normoxic conditions. Hemolymph was collected anaerobically through a cannula after catheterization of the adductor muscle. The mean values of hemolymph pH and Tco2 were 7.442 and 1.50 mM/l, respectively. Using the CO2 solubility coefficient and apparent dissociation constant of carbonic acid determined in this study, Pco2 and [HCO3- ] were calculated as 1.55 mmHg and 1.44 mM/l, respectively. These values were in same range as those of the hemolymph of other marine bivalves of Mytiloida, Pterioida and Ostreoida.