Polypeptone Induces Dramatic Cell Lysis in ura4 Deletion Mutants of Fission Yeast

DOI 機関リポジトリ Web Site PubMed 被引用文献6件 参考文献26件 オープンアクセス

書誌事項

タイトル別名
  • Polypeptone induces dramatic cell lysis in <italic>ura4</italic> deletion mutants of fission yeast
公開日
2013-03-21
資源種別
journal article
DOI
  • 10.1371/journal.pone.0059887
公開者
Public Library of Science

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説明

Polypeptone is widely excluded from Schizosaccharomyces pombe growth medium. However, the reasons why polypeptone should be avoided have not been documented. Polypeptone dramatically induced cell lysis in the ura4 deletion mutant when cells approached the stationary growth phase, and this phenotype was suppressed by supplementation of uracil. To determine the specificity of this cell lysis phenotype, we created deletion mutants of other genes involved in de novo biosynthesis of uridine monophosphate (ura1, ura2, ura3, and ura5). Cell lysis was not observed in these gene deletion mutants. In addition, concomitant disruption of ura1, ura2, ura3, or ura5 in the ura4 deletion mutant suppressed cell lysis, indicating that cell lysis induced by polypeptone is specific to the ura4 deletion mutant. Furthermore, cell lysis was also suppressed when the gene involved in coenzyme Q biosynthesis was deleted. This is likely because Ura3 requires coenzyme Q for its activity. The ura4 deletion mutant was sensitive to zymolyase, which mainly degrades (1,3)-beta-D glucan, when grown in the presence of polypeptone, and cell lysis was suppressed by the osmotic stabiliser, sorbitol. Finally, the induction of cell lysis in the ura4 deletion mutant was due to the accumulation of orotidine-5-monophosphate. Cell wall integrity was dramatically impaired in the ura4 deletion mutant when grown in the presence of polypeptone. Because ura4 is widely used as a selection marker in S. pombe, caution needs to be taken when evaluating phenotypes of ura4 mutants.

収録刊行物

  • PLOS ONE

    PLOS ONE 8 (8), e59887-, 2013-03-21

    Public Library of Science

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参考文献 (26)*注記

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