Metabolic engineering of oleaginous fungus Mortierella alpina for high production of oleic and linoleic acids
この論文をさがす
説明
The aim of this work was to study the molecular breeding of oleaginous filamentous Mortierella alpina for high production of linoleic (LA) or oleic acid (OA). Heterologous expression of the Δ12-desaturase (DS) gene derived from Coprinopsis cinerea in the Δ6DS activity-defective mutant of M. alpina increased the LA production rate as to total fatty acid to 5 times that in the wild strain. By suppressing the endogenous Δ6I gene expression by RNAi in the Δ12DS activity-defective mutant of M. alpina, the OA accumulation rate as to total fatty acid reached 68.0%. The production of LA and OA in these transformants reached 1.44 and 2.76 g/L, respectively, on the 5th day. The Δ6I transcriptional levels of the RNAi-treated strains were suppressed to 1/10th that in the parent strain. The amount of Δ6II RNA in the Δ6I RNAi-treated strain increased to 8 times that in the wild strain.
収録刊行物
-
- Bioresource Technology
-
Bioresource Technology 245 (B), 1610-1615, 2017-06-19
Elsevier
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1050022708921221376
-
- NII論文ID
- 120006763922
-
- NII書誌ID
- AA11522750
-
- ISSN
- 18732976
- 09608524
-
- PubMed
- 28673516
-
- 本文言語コード
- en
-
- 資料種別
- journal article
-
- データソース種別
-
- IRDB
- Crossref
- CiNii Articles
- KAKEN
- OpenAIRE
