Ultraviolet（ UV）-and UV-related cytokine-mediated transcriptional mechanisms of type VII collagen gene（ COL7A1） expression in the skin, with special reference to photoaged skin and anti-aging

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  • Ultraviolet UV and UV related cytokine mediated transcriptional mechanisms of type 7 collagen gene COL7A1 expression in the skin with special reference to photoaged skin and anti aging

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Chronic ultraviolet （UV） radiation results in photoaged skin characterized by deep wrinkle formation. Recent studies suggest that diminishment of anchoring fi brils（ AF）, stabilizing the association of the basement membrane to the underlying dermis, are involved in photoaged skin. Expression of COL7A1, encoding type VII collagen that is a major component of AF, is also decreased in photoaged skin. In this study, we have investigated COL7A1 transcription by UV and UV-related cytokines involved in photoaged skin. Nuclear run-on assay, luciferase assay and gel shift assay revealed that UV and UV-related cytokines（ TNF-α, IL-1β） tissue specifi cally downregulated COL7A1 transcription in epidermal keratinocytes, whereas COL7A1 transcription was upregulated by each modulator in dermal fi broblasts. The responsive element of each modulator was located between nucleotide -524 and -22 of COL7A1 promoter and AP-1 and NF-κB families band to this region. Taken together, these data strongly suggest that the downregulation of COL7A1 transcription in epidermal keratinocytes, main cells expressing COL7A1 in the skin, and subsequent diminishment of AF are involved in photoaged skin. We also have discussed relation between these results and pathophysiology of wrinkle formation, characteristic feature of photoaged skin.

弘前医学. 59(Suppl.), 2007, p.S154-S161

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  • 弘前医学

    弘前医学 59 (Supplement), S154-S161, 2007-11-29

    弘前大学大学院医学研究科・弘前医学会

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