＜Poster＞The role of interleukin-17 in the protective effect of an immunization with clumping factor A against Staphylococcus aureus infection

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  • The role of interleukin 17 in the protective effect of an immunization with clumping factor A against Staphylococcus aureus infection

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Clumping factor A （ClfA） is an adhesin of Staphylococcus aureus, and the residues 40-559 of ClfA （ClfA40-559） compose fi brinogen binding domain. It was reported that antibody to ClfA40-559 plays an important role in the protective eff ects. In this study, we investigated the role of IL-17 in the protective eff ects of an immunization with ClfA40-559 against S. aureus infection. 　Mice immunized or non-immunized with ClfA40-559 were challenged with S. aureus. Bacterial numbers in the organs and survival rates were evaluated. RAW264.7, DC2.4 and spleen cells of naïve and immunized mice were stimulated with ClfA40-559. Cytokine production and mRNA expression in the spleens and cells was determined by ELISA and/or RT-PCR. 　The survival rate was improved and the bacterial numbers in the organs were reduced in the mice immunized with ClfA40-559. ClfA40-559 induced IL-23p19 mRNA expression and IL-6 but not IL-12 production in DC2.4 and RAW264.7 cells. RORγt and IL-17A mRNA expression in the spleen cells of naïve mice was induced. In the spleen cells of immunized mice, ClfA40-559 induced the high production of IL-17 but not IFN-γ or IL-4. IL-17A, IL-6 and CXCL2 mRNA expression was also increased in the organs of immunized and challenged mice. These results suggest that an immunization with ClfA40-559 induces Th17, and that IL-17 contributes to host defense in early phase of S. aureus infection by promoting neutrophil recruitment.

弘前医学. 61(Suppl.), 2010, p.S238-S244

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  • 弘前医学

    弘前医学 61 (Supplement), S238-S244, 2010-07-08

    弘前大学大学院医学研究科・弘前医学会

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