The Dugesia ryukyuensis Database as a Molecular Resource for Studying Switching of the Reproductive System
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The planarian Dugesia ryukyuensis reproduces both asexually and sexually, and can switch from one mode of reproduction to the other. We recently developed a method for experimentally switching reproduction of the planarian from the asexual to the sexual mode. We constructed a cDNA library from sexualized D. ryukyuensis and sequenced and analyzed 8,988 expressed sequence tags (ESTs). The ESTs were analyzed and grouped into 3,077 non-redundant sequences, leaving 1,929 singletons that formed the basis of unigene sets. Fifty-six percent of the cDNAs analyzed shared similarity (E-value<1E -20) with sequences deposited in NCBI. Highly redundant sequences encoded granulin and actin, which are expressed in the whole body, and other redundant sequences encoded a Vasa-like protein, which is known to be a component of germ-line cells and is expressed in the ovary, and Y-protein, which is expressed in the testis. The sexualized planarian expressed sequence tag database (http://planaria.bio.keio.ac.jp/planaria/) is an open-access, online resource providing access to sequence, classification, clustering, and annotation data. This database should constitute a powerful tool for analyzing sexualization in planarians.
収録刊行物
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- Zoological Science
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Zoological Science 24 (1), 31-37, 2007-01
Zoological Society of Japan
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詳細情報 詳細情報について
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- CRID
- 1050282677087584384
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- NII論文ID
- 10018877878
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- NII書誌ID
- AA10545874
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- ISSN
- 02890003
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- HANDLE
- 2433/85330
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- NDL書誌ID
- 8690281
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- PubMed
- 17409714
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
- Integbio
