PLENTY, a hydroxyproline<i>O</i>-arabinosyltransferase, negatively regulates root nodule symbiosis in<i>Lotus japonicus</i>
書誌事項
- タイトル別名
-
- PLENTY a hydroxyproline O-arabinosyltransferase negatively regulates root nodule symbiosis in Lotus japonicus
この論文をさがす
説明
Abstract Legumes can survive in nitrogen-deficient environments by forming root-nodule symbioses with rhizobial bacteria; however, forming nodules consumes energy, and nodule numbers must thus be strictly controlled. Previous studies identified major negative regulators of nodulation in Lotus japonicus, including the small peptides CLAVATA3/ESR (CLE)-RELATED-ROOT SIGNAL1 (CLE-RS1), CLE-RS2, and CLE-RS3, and their putative major receptor HYPERNODULATION AND ABERRANT ROOT FORMATION1 (HAR1). CLE-RS2 is known to be expressed in rhizobia-inoculated roots, and is predicted to be post-translationally arabinosylated, a modification essential for its activity. Moreover, all three CLE-RSs suppress nodulation in a HAR1-dependent manner. Here, we identified PLENTY as a gene responsible for the previously isolated hypernodulation mutant plenty. PLENTY encoded a hydroxyproline O-arabinosyltransferase orthologous to ROOT DETERMINED NODULATION1 in Medicago truncatula. PLENTY was localized to the Golgi, and an in vitro analysis of the recombinant protein demonstrated its arabinosylation activity, indicating that CLE-RS1/2/3 may be substrates for PLENTY. The constitutive expression experiments showed that CLE-RS3 was the major candidate substrate for PLENTY, suggesting the substrate preference of PLENTY for individual CLE-RS peptides. Furthermore, a genetic analysis of the plenty har1 double mutant indicated the existence of another PLENTY-dependent and HAR1-independent pathway negatively regulating nodulation.
The gene responsible for the plenty hypernodulation phenotype in Lotus japonicus was identified. The enzymatic activity and Golgi localization of PLENTY demonstrated its function as a post-translational modification enzyme.
収録刊行物
-
- Journal of Experimental Botany
-
Journal of Experimental Botany 70 (2), 507-517, 2019-01
Oxford University Press
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1050282677526625408
-
- NII論文ID
- 120007128131
-
- NII書誌ID
- AA00697526
-
- ISSN
- 14602431
- 00220957
-
- HANDLE
- 2241/00155414
-
- PubMed
- 30351431
-
- 本文言語コード
- en
-
- 資料種別
- journal article
-
- データソース種別
-
- IRDB
- Crossref
- CiNii Articles
- KAKEN
- OpenAIRE