Bibliographic Information
- Other Title
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- フローサイトメトリー ニ ヨル アポトーシス カイセキ ノ カイリョウホウ
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Abstract
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In cells undergoing apoptosis, the characteristic "ladder" pattern of DNA fragments is observed on agarose gel electrophoresis. Such a pattern of DNA degradation generally serves as a criterion for the apoptosis. However, on agarose gel electrophoresis it is difficult to accurately quantify DNA fragmentation. Therefore, quantification of DNA fragmentation is determined by flow cytometry, DNA fragmentation assay etc. In this paper we improved the methods for the analysis of apoptosis by flow cytometry. As a result, we can obtain much information (quantification of apoptosis and cell cycle analysis etc.) from a single sample (1~3×10 6 cells) with the improved methods. The sample is treated with phosphate-citrate buffer. These methods are very simple and convenient.
Journal
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- 三重大学生物資源学部紀要 = The bulletin of the Faculty of Bioresources, Mie University
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三重大学生物資源学部紀要 = The bulletin of the Faculty of Bioresources, Mie University 20 37-45, 1998-03-31
三重大学生物資源学部
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Details 詳細情報について
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- CRID
- 1050282677914103936
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- NII Article ID
- 110000506889
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- NII Book ID
- AN10073846
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- HANDLE
- 10076/10585
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- NDL BIB ID
- 4476186
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- ISSN
- 09150471
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- Text Lang
- ja
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- Article Type
- departmental bulletin paper
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- Data Source
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- IRDB
- NDL
- CiNii Articles