EXPRESSION OF TELOMERASE ACTIVITY ASSOCIATED WITH TRANSFORMATION OF HUMAN FIBROBLASTS

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Telomerase activity and telomere length in normal and immortalized human fibroblasts were determined. In normal cells, no telomerase activity was detected, but after transformation of these cells with SV40 or ^<60>Co, cells changed into immortal and an apparent telomerase activity expressed. Telomere length of these cells were determined and a distinct shortening of telomeres was observed in one case of transformants,and in other cases, telomere elongation was observed. DNA polymerase α activity was also determined, and high activity was detected in the cells having long telomeres, but low activity was detected in the cells bearing short telomeres. The expression of telomerase may be required as a critical step for tumorgenesis, but telomere length may not be a determinants of malignancy of tumor cells.

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