Cell Polysaccharides of the Bacillus Calmette-Guerin (BCG)

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Defatted BCG cells were extracted by successive treatments with warm water, hot water and alkali solution, and the polysaccharide fractions I, II and III were obtained. Paper electrophoresis showed that Polysaccharides I and II each consisted of one component, while III contained three components. An attempt was made to fractionate this with methanol. The chemical and serological properties of these polysaccharide fractions were studied. In addition to mannose, arabinose and glucosamine, glucose and galactose were identified in the polysaccharides on hydrolysis. The neutral sugars were determined by using ion exchange chromatography. The polysaccharide fraction having a high serological activity showed a high hexosamine content.

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