Generation of scaffoldless hyaline cartilaginous tissue from human iPSCs.

DOI IR (HANDLE) HANDLE Web Site Web Site View 1 Remaining Hide 65 Citations 35 References Open Access

Bibliographic Information

Other Title
  • Generation of scaffoldless hyaline cartilaginous tissue from human iPSCs [in English]

Search this article

Description

Defects in articular cartilage ultimately result in loss of joint function. Repairing cartilage defects requires cell sources. We developed an approach to generate scaffoldless hyaline cartilage from human induced pluripotent stem cells (hiPSCs). We initially generated an hiPSC line that specifically expressed GFP in cartilage when teratoma was formed. We optimized the culture conditions and found BMP2, transforming growth factor β1 (TGF-β1), and GDF5 critical for GFP expression and thus chondrogenic differentiation of the hiPSCs. The subsequent use of scaffoldless suspension culture contributed to purification, producing homogenous cartilaginous particles. Subcutaneous transplantation of the hiPSC-derived particles generated hyaline cartilage that expressed type II collagen, but not type I collagen, in immunodeficiency mice. Transplantation of the particles into joint surface defects in immunodeficiency rats and immunosuppressed mini-pigs indicated that neocartilage survived and had potential for integration into native cartilage. The immunodeficiency mice and rats suffered from neither tumors nor ectopic tissue formation. The hiPSC-derived cartilaginous particles constitute a viable cell source for regenerating cartilage defects.

Journal

Citations (65)*help

See more

References(35)*help

See more

Related Projects

See more

Keywords

Details 詳細情報について

Report a problem

Back to top