Purification Method of Proteins by Preparative Gel Electrophoresis. Crystallisation of α-amylases.

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説明

Taka-amylase A from Aspergillus orizae and huma α-amylase were crystallized in a three-step procedure: (1) Crude extract o fTaka-diastase (Sankyo) or saliva supernatant was applied to a gel slab type apparatus of preparative electrophoresis. The active fractions were extracted by crushing the gel slices with a buffer solution.(2) The soluble gel components, which were extracted from the gel slices together with protein fractions, were removed by electrophoresis with a discontinuous buffer system containing sucrose gradient (purification electrophoresis).(3) After acetone fractionation, crystalline amylases were obtained. The overall recovery rates of Taka-amylase A and human salivary amylase were 51% and 53%, respectively. Further usage of preparative gel electrophoresis and purification electrophoresis to obtaining pure proteins was examined.

収録刊行物

  • 山口医科大学紀要

    山口医科大学紀要 23 (3-4), 165-183, 1976-12

    Yamaguchi University Graduate School of Medicine

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詳細情報 詳細情報について

  • CRID
    1050282812427310592
  • NII論文ID
    120000864147
  • Web Site
    https://petit.lib.yamaguchi-u.ac.jp/2089
  • 本文言語コード
    en
  • 資料種別
    departmental bulletin paper
  • データソース種別
    • IRDB
    • CiNii Articles

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