ZnCl2 and vitamin C, known as antioxidants, differently potentiate the cytotoxicity of H2O2 in rat thymocytes : Cytometric analysis using forward and side scatters
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Abstract
The ‘antioxidant hypothesis’ proposes that antioxidant nutrients afford protection against chronic diseases by decreasing oxidative damages. The ability of zinc to retard oxidative processes has been recognized for many years. However, the application of ZnCl2 potentiates the cytotoxicity of H2O2. Thus, some antioxidants may be cytotoxic under certain oxidative conditions. Therefore, in this study, the effect of vitamin C, one of antioxidant nutrients, on the cells treated with H2O2 has been examined to see if vitamin C potentiates the cytotoxicity of H2O2. Experiments were carried out with flow cytometer and rat thymocytes. Vitamin C also potentiated the cytotoxicity of H2O2. The increase in cell lethality induced by the combination of H2O2 and ZnCl2 was associated with the increase in population of shrunken cells with increased intensity of side scatter. However, it was not the case for the combination of H2O2 and vitamin C. The profile of cytotoxicity induced by H2O2 and vitamin C was different from that by H2O2 and ZnCl2. It may be suggested that the effects of zinc and vitamin C varies from cytoprotective to cytotoxic, being dependent on the type of oxidative stress.
Journal
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- 徳島大学総合科学部自然科学研究 = Natural Science Research, The University of Tokushima
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徳島大学総合科学部自然科学研究 = Natural Science Research, The University of Tokushima 24 (3), 13-20, 2010-07-20
[徳島] : 徳島大学総合科学部 ; 1988-2015
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Details 詳細情報について
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- CRID
- 1050282812440419968
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- NII Article ID
- 110008703796
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- NII Book ID
- AN10065859
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- ISSN
- 09146385
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- NDL BIB ID
- 023446514
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- Text Lang
- en
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- Article Type
- departmental bulletin paper
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- Data Source
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- IRDB
- NDL
- CiNii Articles