Role of Zn2+ in restoration of nonprotein thiol content in the cells under chemical stress induced by triclocarban
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説明
We have proposed that intracellular Zn2+ release during oxidative stress is a trigger to restore cellular thiol content that is decreased by oxidative stress. Recently, we found that the incubation with triclocarban for 1 h decreased cellular thiol content and increased intracellular Zn2+ concentration. It was reminiscent of the possibility that the increase in intracellular Zn2+ concentration by triclocarban could become a trigger to restore the cellular content of nonprotein thiols. To test the possibility, we cytometrically examined the effects of prolonged incubation (3 h) with triclocarban on the cellular content of nonprotein thiols, presumably glutathione, by the use of 5-chloromethylfluorescein (5-CMF) diacetate, a fluorescent indicator of cellular nonprotein thiols, in rat thymocytes. The intensity of 5-CMF fluorescence after the 3 h incubation with 300 nM triclocarban was significantly higher than that after the 1 h incubation. In the presence of a chelator of intracellular Zn2+, such a significant difference was not observed. The results suggest that the increase in intracellular Zn2+ concentration by triclocarban is one of triggers to restore cellular content that is decreased by triclocarban.
収録刊行物
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- 徳島大学総合科学部自然科学研究 = Natural Science Research, The University of Tokushima
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徳島大学総合科学部自然科学研究 = Natural Science Research, The University of Tokushima 27 (1), 1-5, 2013-01
[徳島] : 徳島大学総合科学部 ; 1988-2015
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詳細情報 詳細情報について
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- CRID
- 1050282812441551488
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- NII論文ID
- 110009915516
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- NII書誌ID
- AN10065859
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- ISSN
- 09146385
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- NDL書誌ID
- 026939136
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- 本文言語コード
- en
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- 資料種別
- departmental bulletin paper
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- データソース種別
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- IRDB
- NDL
- CiNii Articles