Efficacy of Recombinant Canine Distemper Virus Expressing Leishmania Antigen against Leishmania Challenge in Dogs
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- Ricardo Toshio Fujiwara
- editor
書誌事項
- 公開日
- 2015-07
- 資源種別
- journal article
- 権利情報
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- Copyright: © 2015 Miura et al.
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
- https://creativecommons.org/licenses/by/4.0/ | https://creativecommons.org/licenses/by/4.0/
- DOI
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- 10.1371/journal.pntd.0003914
- 公開者
- Public Library of Science
説明
Canine distemper virus (CDV) vaccination confers long-term protection against CDV reinfection. To investigate the utility of CDV as a polyvalent vaccine vector for Leishmania, we generated recombinant CDVs, based on an avirulent Yanaka strain, that expressed Leishmania antigens: LACK, TSA, or LmSTI1 (rCDV–LACK, rCDV–TSA, and rCDV–LmSTI1, respectively). Dogs immunized with rCDV-LACK were protected against challenge with lethal doses of virulent CDV, in the same way as the parental Yanaka strain. To evaluate the protective effects of the recombinant CDVs against cutaneous leishmaniasis in dogs, dogs were immunized with one recombinant CDV or a cocktail of three recombinant CDVs, before intradermal challenge (in the ears) with infective-stage promastigotes of Leishmania major. Unvaccinated dogs showed increased nodules with ulcer formation after 3 weeks, whereas dogs immunized with rCDV–LACK showed markedly smaller nodules without ulceration. Although the rCDV–TSA- and rCDV–LmSTI1-immunized dogs showed little protection against L. major, the cocktail of three recombinant CDVs more effectively suppressed the progression of nodule formation than immunization with rCDV–LACK alone. These results indicate that recombinant CDV is suitable for use as a polyvalent live attenuated vaccine for protection against both CDV and L. major infections in dogs.
収録刊行物
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- PLoS Neglected Tropical Diseases
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PLoS Neglected Tropical Diseases 9 (7), e0003914-1-e0003914-15, 2015-07
Public Library of Science
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キーワード
詳細情報 詳細情報について
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- CRID
- 1050282812465476608
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- NII論文ID
- 120006311882
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- ISSN
- 19352735
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- HANDLE
- 10659/4955
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- PubMed
- 26162094
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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