Phytophthora capsiciの遊走子の運動に関する研究-5-各種緩衝液中における遊泳異常と前・後鞭毛の働き

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  • Phytophthora capsici の遊走子の運動に関する研究 V : 各種緩衝液中における遊泳異常と前・後鞭毛の働き(農学部門)
  • Phytophthora capsici ノ ユウソウコ ノ ウンドウ ニ カンスル ケンキュウ 5 カクシュ カンショウエキ チュウ ニ オケル ユウエイ イジョウ ト マエ アト ベンモウ ノ ハタラキ
  • Movements of zoospores of Phytophthora capsici V. : Abnormal swimming of zoospores in some buffer solutions and the functions of anterior and posterior flagella (Agriculture)

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Abnormal swimmings of zoospores of Phytophthora capsici Leon. were observed in some buffer solutions under the dark-field illumination, and assumption was made as to the functions of anterior and posterior flagella in swimming of zoospores. In Sorensen phosphate buffer (buffer/zoospore suspension, B/Φ=3/1), at pH 5.5,zoospores showed snakey movement (Fig. 1-1), and their rotation became gradually imparfect. Finally zoospores entered in a smooth circular swimming without rotating (Fig. 1-2). At pH 8.0,zoospores swam straightly with rotation at the beginning (Fig. 1-3), but the most of them showed irregular circular movement at the later stage (Fig. 1-4). These observation suggests that the posterior flagellum was functionally obstructed at pH 5.5,while the anterior flagellum lost its function before the posterior one at pH 8.0. The functional obstruction of zoospore swimming was better elucidated in Michaelis buffer (B/Φ=2/2) than in Sorensen buffer. At pH 5.5,undulation of anterior flagellum with a large amplitude was observed just before the cease of spore movement. Many of anterior flagellum-zoospores were recognized at pH 5.5 and 6.5,and posterior flagellum-zoospores at pH 8.0. The former swam smoothly in circle, but the later showed on irregular spiral path or unoven circle. In McIlvaine buffer (B/Φ=1/3), zoospores swam straightly with rotation regardless of pH values. Swimming velocities were slower than normal, but the frequency of rotation was almost the same. From these observations the following assumption was made as to the mode. The zoospores swim in a spiral path rotating on their axis. The two flagella are attached laterally, one projecting forward, the other backward. Each flagellum undulates in only one plane with respect to the zoospore. Both are active in propelling the spore. The spores rotate on their axis, and this rotation keeps the straight swimming of the spore even though the flagella are attached at one side of the cell. The posterior flagellum is mainly responsible for the rotation of spore, and the anterior one supports it by keeping the balance of body.

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