Morphological and Cytochemical Characteristics of Neutrophil from African Lungfish, Protopterus annectens

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  • アフリカハイギョProtopterus annectens好中球の形態学的および細胞化学的特徴
  • アフリカハイギョ　Protopterus annectens　コウチュウキュウ　ノ　ケイタイガクテキ　オヨビ　サイボウカガクテキ トクチョウ

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Morphological and cytochemical characteristics of neutrophil from African lungfish, Protopterus annectens, were examined by light microscopy. The neutrophils were round to oval (35-40μm in diameter) and the nucleus round to lobule-shaped. Only one type of granule, panchromatophilic granule (PG) was observed in the neutrophil. The PG was round to rod-shaped and stained panchromatophilic according to staining conditions. The PG was eosinophilic or methylenophilic（methylene blue positive) with May-Gr?nwald (MG). And further, the PG showed reddish purple with Giemsa stain. This color tone may be due to the metachromatic reaction by azure B in Giemsa (metaazurophilic). This granule was acid phosphatase, α-naphtyl acetate esterase, α-naphtyl butyrate esterase, naphthol AS-D chloroacetate esterase, PAS (α-amylase digestion resistance) and toluidine blue (metachromatic) positive.

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