In Vitro Antimicrobial Activity of Green Synthesized Silver Nanoparticles Against Selected Gram-negative Foodborne Pathogens
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- Loo, Yuet Ying
- Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia
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- Rukayadi, Yaya
- Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia
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- Nor-Khaizura, Mahmud-Ab-Rashid
- Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia
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- Kuan, Chee Hao
- Department of Agricultural and Food Science, Faculty of Science, Universiti Tunku Abdul Rahman
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- Chieng, Buong Woei
- Department of Chemistry, Faculty of Science, Universiti Putra Malaysia・Materials Processing and Technology Laboratory, Institute of Advanced Technology, Universiti Putra Malaysia
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- Nishibuchi, Mitsuaki
- Center for Southeast Asian Studies, Kyoto University
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- Radu, Son
- Department of Food Science, Faculty of Food Science and Technology・Laboratory of Food Safety and Food Integrity, Institute of Tropical Agriculture and Food Security (ITAFoS), Universiti Putra Malaysia
Description
Silver nanoparticles (AgNPs) used in this study were synthesized using pu-erh tea leaves extract with particle size of 4.06 nm. The antibacterial activity of green synthesized AgNPs against a diverse range of Gram-negative foodborne pathogens was determined using disk diffusion method, resazurin microtitre-plate assay (minimum inhibitory concentration, MIC), and minimum bactericidal concentration test (MBC). The MIC and MBC of AgNPs against Escherichia coli, Klebsiella pneumoniae, Salmonella Typhimurium, and Salmonella Enteritidis were 7.8, 3.9, 3.9, 3.9 and 7.8, 3.9, 7.8, 3.9 μg/mL, respectively. Time-kill curves were used to evaluate the concentration between MIC and bactericidal activity of AgNPs at concentrations ranging from 0×MIC to 8×MIC. The killing activity of AgNPs was fast acting against all the Gram-negative bacteria tested; the reduction in the number of CFU mL⁻¹ was >3 Log₁₀ units (99.9%) in 1–2 h. This study indicates that AgNPs exhibit a strong antimicrobial activity and thus might be developed as a new type of antimicrobial agents for the treatment of bacterial infection including multidrug resistant bacterial infection.
Journal
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- Frontiers in Microbiology
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Frontiers in Microbiology 9 2018-07-16
Frontiers Media SA
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Keywords
Details 詳細情報について
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- CRID
- 1050282813185968384
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- NII Article ID
- 120006554788
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- ISSN
- 1664302X
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- HANDLE
- 2433/236376
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- Text Lang
- en
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- Article Type
- journal article
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- Data Source
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- IRDB
- CiNii Articles