The Yagi's Perfusion Method for Frog Heart Improved to Enable Quantitative Experiments: From the Measurement of Cardiac Output to Simultaneous ECG Recording

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  • ｶｴﾙ心臓八木式灌流法の定量的実験への改良 ―心拍出量測定から心電図同時記録まで―
  • カエル シンゾウ ヤギシキカンリュウホウ ノ テイリョウテキ ジッケン エ ノ カイリョウ : シンパクシュツリョウ ソクテイ カラ シンデンズ ドウジ キロク マデ

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The Yagi's perfusion system has widely been used to study the beats of excised frog heart. Here, we describe a new method to obtain a measure of cardiac output of the frog heart perfused with the Yagi's system. In the new method, the changes in ventricular volume during systole and diastole are converted to the changes in air pressure by using the communicating vessel chamber in which the tissue chamber bathing the ventricle is connected via a communicating tube to the air-tight chamber for pressure detection. With this method, the Frank-Starling mechanism associated with the changes in preload is clearly demonstrable. With an additional apparatus to increase the hydrostatic pressure within the aorta, it is possible to show the unique changes in ventricular dynamics accompanying the increases in afterload. In addition, a method to stably record the ECG simultaneously with the above measurement is described. Our new methods may be useful for the student practice in which students should learn the physiological as well as pharmacological properties of heart.

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