Synthesis of ρ-Nitrophenyl β-D-Gentiobioside by Streptomyces β-Glucosidase

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  • 放線菌β-グルコシダーゼによるρ-ニトロフェニルβ-D-ゲンチオビオシドの合成
  • ホウセンキン ベータ グルコシダーゼ ニ ヨル p ニトロフェニル ベータ D

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Abstract

P(論文)

Streptomyces β-glucosidase was found to catalyze the transglucosylation from ρ-nitro-phenyl β-D-glucoside (PNPG). A main product formed was purified by HP-20 column chromatogrphy follwing to TOYOPEARL HW-40F column chromatography. The transfer product obtained was identified as ρ-nitrophenyl β-D-gentiobioside (PNPGen) with the enzymatic and acid hydrolysis, and methylation analysis. This result was supported by MS and ^<13>C-NMR.

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