May-Grűnwald • Giemsa Staining Properties of Granules in Tilapia Oreochromis niloticus Neutrophil

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  • テラピラ好中球顆粒のメイ-グリュンワルド・ギムザ染色性
  • テラピア　コウチュウキュウ　カリュウ　ノ　メイ　グリュンワルド　ギムザ　センショクセイ
  • May-grunwald giemsa staining properties of granules in tilapia Oreochromis niloticus neutrophil
  • テラピア好中球顆粒のメイ-グリュンワルド・ギムザ染色性

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May-Grunwald . Giemsa (MGG) staining properties of cytoplasmic granules in tilapia Oreochromis niloticus neutrophil were examined in this study. Four types of granules; eosinophilic granule (αG), chromophobic (neutrophilic) granule (βG), azurophilic (basophilic) granule (γG) and curious basophilic granule (δG) were observed in neutrophil. The staining pattern of αG and γG was strongly influenced by pH and concentration of diluent of the staining solution. The αG was small (<0.3 μm in diameter) and round in shape. Eosinophil of αG was disappeared by long-time Giemsa stain after May-Grunwald (MG). The βG was round to oval and was larger (0.5~1.0μm in diameter) than αG. The γG was round to oval (<0. 3 μm in diameter), and observed in MGG and in Giemsa staining preparations (distilled water and neutral buffer as diluent) and in MG using buffer (pH6 and 7). The δG was basophilic, larger than γG, irregular in shape (rod, comma, crescent, ring) and observed in 50-80% of neutrophil. This granule was not found in MGG and MG using acid buffer (pH5).

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