Infectivity Analysis of the Cloned Component of a Monopartite Begomovirus-Betasatellite Complex Causing Tomato Leaf Curl Disease in Pakistan

JASI Web Site

Bibliographic Information

Other Title
  • パキスタンに発生したトマト巻葉病を引き起こす単一ゲノム性Begomovirusおよびβサテライトの感染性クローンによる感染性解析

Search this article

Abstract

Cotton leaf curl Rajasthan virus (CLCuRaV), a monopartite begomovirus causing devastating losses of cotton crop in India, was isolated from tomato (Solanum lycopersicum) along with DNA satellite (CLCuRaB sup(tomato)) and characterized in Faisalabad, Pakistan. Infectious clones of CLCuRaV sup(tomato) and CLCuRaB sup(tomato) were produced and transformed into Agrobacterium strain GV3101. A partial tandem repeat construct of CLCuRaV sup(tomato) was inoculated to Nicotiana benthamiana and tomato plants alone and/or with CLCuRaB sup(tomato). CLCuRaV sup(tomato) alone produced very mild symptoms in N. benthamiana plants, whereas tomato plants showed no symptoms by inoculation with CLCuRaV sup(tomato) in the absence of CLCuRaB sup(tomato). In N. benthamiana plants 100% infection followed by severe symptoms was observed while in tomato plants 70-80% infection with mild symptoms was observed on co-inoculation with CLCuRaV sup(tomato) and CLCuRaB sup(tomato). Symptoms severity of CLCuRaV sup(tomato) increased in the presence of CLCuRaB sup(tomato) in N. benthamiana and tomato plants. Here we demonstrated the first experimental pathogenicity analysis of a cotton virus isolated from tomato plants both in N. benthamiana and tomato plants.

Journal

Keywords

Details 詳細情報について

Report a problem

Back to top