Genomic Structure and Expression of the Soluble Guanylyl Cyclase α<sub>2</sub>Subunit Gene in the Medaka Fish Oryzias latipes

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  • Genomic Structure and Expression of the Soluble Guanylyl Cyclase α2 Subunit Gene in the Medaka Fish Oryzias latipes
  • Genomic structure and expression of the soluble guanylyl cyclase アルファ 2 subunit gene in the medaka fish Oryzias latipes

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A cDNA clone encoding the soluble guanylyl cyclase α2 subunit was isolated from medaka fish (Oryzias latipes) and designated as OIGCS-α2. The OIGCS-α2 cDNA was 3192 bp in length and the open reading frame (ORF) encodes a protein of 805 amino acids. The deduced amino acid sequence has high similarity to that of the mammalianα2 subunit gene except for the N-terminal regulatory domain. The C-terminal 5 amino acids, "RETSL", which have been reported to interact with the post synaptic density protein (PSD)-95 were conserved. An RNase protection assay with adult fish organs showed that O/GCS-α2 was expressed mainly in the brain and testis. The complete nucleotide sequence (about 41 kbp) of the OIGCS-α2 genomic DNA clone isolated from a medaka fish BAC library indicated that the OIGCS-α2 gene consisted of 9 exons and 8 introns. The 5'-flanking region and larger introns, such as introns 1, 4, and 7, contained the several fragments conserved in the nucleotide sequences of Rex6 (non-long terminal repeat retrotransposon), MHC class I genomic region, and OIGC1, the medaka fish homolog of the mammalian guanylyl cyclase B gene. Linkage analysis on the medaka fish chromosome demonstrated that the OIGCS-α2 gene was mapped to LG13; this mapping position was different from those for the OIGCS-α1 and OIGCS-β1 genes (LG1).

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