ウニ病害原因菌 Vibrio sp. の同定のための 16S rRNA 遺伝子を標的にした特異的 PCR の開発

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タイトル別名
  • Development of 16S rRNA targeted PCR for the identification of Vibrio spp., the causative bacteria of the discase in cultured sea urchin Stronglylocentrotus intermedius occurring at low water temperatures

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The causative bacteria, Vibrio spp., have been isolated from the diseased sea urchin Strongylocentrotus intermedius at low water temperatures. To develop a specific identification method for the pathogens, species-specific nucleotide sequences in the 16S rRNA of Vibrio spp., were determined. We designed two PCR primers, DA2F and SK1F, based on the nucleotide sequences : DA2F forward primer (nucleotide numbers 456 to 476 in Escherichia coli 16S rRNA) for Date isolates and Sk1F forward primer (nucleotide numbers 455 to 478 in E. coli 16S rRNA) for Shiriuchi and Shikabe isolates. The PCR product with about 1kbp was obtained in all causative bacteria from Date, Shiriuchi, Shikabe Breeding Centers and some of Vibrio reference strains by amplification with DA2F-1540R primer set, however, those from three Breeding Centers differed from the reference strains in the case of growth at 30℃. PCR amplification with SK1F-1540R primer set was able to differentiate the causative bacteria from Date and those from Shiriuchi and Shikabe. These results indicate that PCR amplification with two primers is useful for identification of Vibrio spp., which are the pathogens of cultured sea urchin.

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詳細情報 詳細情報について

  • CRID
    1050282813978965376
  • NII書誌ID
    AN00193422
  • HANDLE
    2115/42513
  • ISSN
    00215392
  • 本文言語コード
    ja
  • 資料種別
    journal article
  • データソース種別
    • IRDB

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