甲状腺ホルモン応答配列を組換えたレポータープラスミドを用いた遺伝子活性試験システムについて

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  • コウジョウセン ホルモン オウトウ ハイレツ オ クミ カエタ レポータープラスミド オ モチイタ イデンシ カッセイ シケン システム ニ ツイテ
  • Gene Activation Assay System Using Reporter Plasmid with Thyroid Hormone Responsive Element

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Abstract

RXR/TR reporter plasmid for gene activation assay was produced using recombinant DNA techniques. Thyroid hormone responsive element nucleotide sequences （AGGTTC tttcAGGTTC） was inserted into a reporter plasmid （pGL3 vector）. After transfection of RXR/TR reporter plasmid into HepG2 cells, the activities of luciferin-luciferase were determined on various concentrations of thyroid hormone （T3）. This reporter plasmid gene activation assay was highly sensitive to allow the detection of T3 effects in HepG2 cell, detecting the effect of 10-8 to 10-4 M thyroid hormone. Further experiments using the RXR/TR reporter plasmid gene activation assay system will be conducted in order to detect of thyroid hormone like-compounds in environmental pollutants.

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