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Quantitative analysis of Rice stripe virus in a transovarial transmission cycle during the development and reproduction of its vector, Laodelphax striatellus

IR

Abstract

The amount of Rice stripe virus (RSV) maintained through transovarial transmission was analyzed during the development and reproduction of its vector, Laodelphax striatellus. Reverse transcription–quantitative PCR analysis was used to quantify RNA expressed from the RSV coat protein (CP) gene as an estimate of RSV content in nymphs and adults of L. striatellus at various developmental stages. The 18S ribosome RNA gene of L. striatellus was chosen as the reference for calculating RSV CP expression by using the comparative Ct method. Based on the CP transcript levels, the amount of RSV did not differ significantly throughout the nymphal stage or between adult females of different ages; however, RSV content tended to increase slightly as males became older. The average RSV content in males was 1.30 to 2.49 times that in females. The amount of RSV in L. striatellus adults was compared between generations. The RSV content of female adults did not differ significantly between the parent and progeny populations three of three different females. L. striatellus grown to adults on a susceptible cultivar and five RSV-resistant cultivars were compared to analyze whether the amount of RSV varied among cultivars. Although the amount of RSV in L. striatellus adults differed significantly among the six rice cultivars evaluated, the difference seemed independent of whether resistance genes were present. In addition, the percentage of viruliferous insects was similar among cultivars.

source:SC10201804160190

source:NARO成果DBa

source:author manuscript

source:このアーカイブは著者版です。出版社版ではありません。引用の際には出版社版をご利用ください。

source:This is not the published version. Please cite only the published version.

source:This is a post-peer-review, pre-copyedit version of an article published in Virus Genes.

source:The final authenticated version is available online at: http://dx.doi.org/10.1007/s11262-017-1473-8

source:https://doi.org/10.1007/s11262-017-1473-8

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Details

  • CRID
    1050286428524791168
  • NII Article ID
    120006900536
  • ISSN
    09208569
  • Web Site
    http://id.nii.ac.jp/1578/00002598/
  • Text Lang
    en
  • Article Type
    journal article
  • Data Source
    • IRDB
    • CiNii Articles

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