As the method for judging the physiological and environmental abnormality of fish, in the same way as the former report, the differences of hematocrit (Ht) and erythrocyte sedimentation rate (E.S.R.) between the bloods sampled firstly and secondly from the treated carps were compared with those between the bloods sampled firstly and secondly from the intact as control. 1. By the first blood drawing of 1/200～1/1200 (cc./g.) of body weight, Ht decreased and E.S.R. increased in the second blood drawing after one day of no feeding. 2. Ht of the bloods drawn secondly from, the normal carps decreased more than that of the bloods drawn firstly, E.S.R. of the former decreased more than that of the latter. 3. By the injection of heavy metal salts 0.1～0.2cc. of 0.5～1.0ppm. solution into carp muscle immediately after the first blood drawing, Ht and E.S.R. of the second blood drawing (after one or two days of no feeding) varied significantly. Namely, by the injection of mercuric chloride (0.2cc. of 1ppm) Ht increased remarkably, but E.S.R. decreased (after one day); by that of 0.5ppm solution Ht increased or varied at all and E.S.R. varied but little (after one day); by that of cupric chloride (0.2cc.of 1.0ppm.) Ht. increased but E.S.R. decreased (after one day); by that of lead chloride (0.2cc. of 1.0ppm.) Ht increased significantly but E.S.R. decreased; by that of cupric sulphate (0.1cc. of 1.0ppm.) Ht and E.S.R. varied little (after two days). 4. When carps were reared in the solution of heavy metal salts of 0.1～0.5ppm. for two or four days of no feeding, in case of mercuric chloride (0.5ppm. for two days) Ht increased a little in average but E.S.R. varied at all; in case of cupric sulphate (0.5ppm. for two days) the former increased a little but the latter decreased a little in average; in case of lead chloride (0.1ppm. for four days) the former increased a little but the latter varied little in average. 5. It was presumed from these results that by the in jection or treatment of the solution of heavy metal salts, the loss of blood water, the decrease of erythrocytes count caused by check of blood production and the decrease of oxygen consumption were brought about.

魚類血液の量的変化から魚体の生理的・環境的異常を判定する方法として,前報12)と全様に,第1回採血時と第2回採血時のHt:E.S.R.の変化を対照と比較して,重金属塩類の魚類血液えの影響を比較した.材料は全て130g以上200g位までの満1年の養殖ゴイである.1.体重の1/200から1/1200(C.C/g)までの第1回目採血量では,24時間無投餌放養後の第2回目採血に於て,全てHtは第1回目より減少しE.S.R.は増加する.2.普通魚のHtは,第2採血時には第1採血時より減少しE.S.R.は増加する.3.重金属塩類の0.5～1.Oppm.,0.2C.Cの筋肉注射実験では(無投餌で1～2日後),HgCl2の注射(1.Oppm.,1日後)でHtは著増しE.S.R.は減少し,HgCl2の注射(0.5ppm.,1日後)ではHtの増加する時も変化しない時もあり,E.S.R.には余り変化がなく,CuCl2(1.Oppm.,1日後)ではHtは増加しE.S.Rは減少し,PbCl2(1.Oppm.,1日後)ではHtは著増しE.S.R.は減少し,CuSO4(1.Oppm.,0.1C.C.,2日後)では,HtにもE.S.R.にも殆んど影響がなかった.4.重金属塩類の0.1～0.5ppm.飼水内に2～4日間無投餌で放養すると,HgCl2(0.5ppm.中2日間)ではHtは平均して少し増加し,E.S.R.は変化がな,CuSO4(0.5ppm.中2日間)ではHtは平均して少し増加しE.S.R.は少し減少し,PbCl2(O.lppm.中4日間)ではHtは平均して少し増加し,E.S.R.も平均して殆んど変化がなった.5.重金属塩類の筋肉注射(0.5～1.Oppm.,0.2C.C)も飼水添加(0.1～0.5ppm.)も1～4日後には,血液水分の喪失,造血機能阻害による赤血球数の減少,従って酸素消費量の減少を招来するものと推定される.

長崎大学水産学部研究報告, v.14, pp.43-52; 1963