人工的悪性細胞の作製とその評価について
書誌事項
- タイトル別名
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- Production of artificial malignant cells and their evaluation
説明
The transcription factor Snail is involved in gastrula invasion, organogenesis, and cancer cell invasion/ metastasis during early embryogenesis. Snail also has the function of inducing epithelial-mesenchymal transition (EMT). The author introduced a Snail gene expression vector into human colon cancer cell line DLD-1 cells to prepare Snail overexpression cells (called DLD-1/Snail cells). DLD-1/Snail cells changed from epithelial-like morphology to mesenchymal cell morphology and decreased proteins levels such as epithelial makers and increased migration and invasion, inducing EMT. As a result of microarray analysis, the expression of α-1,3/4 fucosyltransferase (Fuc-TⅢ) was decreased in DLD-1/Snail cells compared with control cells. Fuc-TⅢ is a glycosyltransferase required to produce the cancer-related chain antigen sialyl-lewis a (sLea). It was a ligand that adhesive to vascular endothelial cells via E-selectin. It has been suggested that sLea is involved in cancer cell metastasis. However, decreased expression of Fuc-TⅢ in DLD-1/Snail cells reduced sLea antigens. The above results found that during EMT induction by Snail, the cells suppressed the fucosyltransferase expression and eliminated the cell surface's sLea antigen.
収録刊行物
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- 宇部工業高等専門学校研究報告
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宇部工業高等専門学校研究報告 67 7-12, 2021-03
宇部工業高等専門学校
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詳細情報 詳細情報について
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- CRID
- 1050287673833761024
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- NII論文ID
- 120007008488
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- 本文言語コード
- ja
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- 資料種別
- departmental bulletin paper
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- データソース種別
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- IRDB
- CiNii Articles
