Ca
            <sup>2+</sup>
            activity maps of astrocytes tagged by axoastrocytic AAV transfer

Leonidas, Georgiou, Anaí, Echeverría, Achilleas, Georgiou, Bernd, Kuhn

doi:10.1126/sciadv.abe5371

Astrocytes exhibit localized Ca2+ microdomain (MD) activity thought to be actively involved in information processing in the brain. However, functional organization of Ca2+ MDs in space and time in relationship to behavior and neuronal activity is poorly understood. Here, we first show that adeno-associated virus (AAV) particles transfer anterogradely from axons to astrocytes. Then, we use this axoastrocytic AAV transfer to express genetically encoded Ca2+ indicators at high-contrast circuit specifically. In combination with two-photon microscopy and unbiased, event-based analysis, we investigated cortical astrocytes embedded in the vibrissal thalamocortical circuit. We found a wide range of Ca2+ MD signals, some of which were ultrafast (≤300 ms). Frequency and size of signals were extensively increased by locomotion but only subtly with sensory stimulation. The overlay of these signals resulted in behavior-dependent maps with characteristic Ca2+ activity hotspots, maybe representing memory engrams. These functional subdomains are stable over days, suggesting subcellular specialization.

source:https://www.science.org/doi/10.1126/sciadv.abe5371

Ca <sup>2+</sup> activity maps of astrocytes tagged by axoastrocytic AAV transfer

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