ヒト肝臓の脂肪酸の研究 : 1.ヒト肝臓脂肪酸の部分的同定

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  • ヒト肝臓の脂肪酸の研究-1-ヒト肝臓脂肪酸の部分的同定〔英文〕
  • ヒト カンゾウ ノ シボウサン ノ ケンキュウ 1 ヒト カンゾウ シボウサン ノ ブブンテキ ドウテイ エイブン
  • STUDIES ON FATTY ACIDS OF HUMAN LIVER. : 1. PARTIAL CHARACTERIZATION OF FATTY ACIDS OF HUMAN LIVER
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ヒト肝臓の脂肪酸のガスクロマトグラフィーを行ない, 1)標準物質のクロマトグラムとの比較, 2)水素化物のガスクロマトグラフィー, 3)硝酸銀薄層クロマトグラフィーによる分画, 4)質量分析法などの成績とあわせて,飽和-10,モノ不飽和-8,ジ不飽和-5を含む35の脂肪酸を同定した。

The fatty acids of human liver were analyzed as their methyl esters by gas-liquid chromatography. Thirty five fatty acids, including 10 saturated, 8 monoenoic, 5 dienoic acids, and other polyunsaturated fatty acids, were characterized by the following methods: (1) comparison of their chromatographic characteristics with those of standards, (2) hydrogenation for carbon chain length analysis, (3) thin-layer chromatography on silica gel impregnated with silver nitrate for the analysis of unsaturation. (4) The molecular weight of polyunsaturated fatty acids were determined by mass-spectrometry coupled with gas-liquid chromatography.

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