REAL-TIME MONITORING OF DISSOLVED OXYGEN QUENCHED FLUORESCENCE AND MATERIALS MOVEMENTS INDUCED BEAM DEFLECTION AT A VICINITY OF AN AQUATIC PLANTS

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A novel optical detection system was developed to achieve real-time and simultaneous monitoring of dissolved oxygen (DO) quenched-fluorescence and materials movement induced-beam deflection at a vicinity of an aquatic plant surface. A laser beam of 470 nm was passed through the vicinity of the aquatic plant in a culture dish filled with a culture solution containing 10-6 M fluorescent Tris (2, 2'-bipyridyl) ruthenium (II) chloride. Deflection of the laser beam and fluorescence excited by the laser were recorded simultaneously. Experimental results showed that only a little ruthenium complex might be adsorbed on the aquatic plant. Also, romoving DO for obtaining fluorescence intensity without DO was studied. It was demonstrated that the optical detection system could be used for real-time in situ monitoring of the DO-quenched fluorescence and materilas movement-induced beam deflection at the same time.

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